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Chapter 4: Hybrid molecules

Concept of hybrid molecules:
When double strand DNA is steamed to a temperature
exceed the melting temperature (Tm), it will separate
into 2 single strands DNA due to breaks of H bonds. If
the reaction temperature is then decreased slowly plus
other appropriate experimental conditions, these
ssDNA will pair again. This phenomenon is called the
hybridization of molecules.
Characteristic of the hybrid molecules: Specificity, the
re-pairing occurs only between two complementary
sequences.

Từ khóa: the hybrid molecules, phenomenon is called, types of hybridization, single strands DNA, the reaction temperature, concept of hybrid molecules

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